Overview
Product name | Recombinant Human GSTA1 Protein |
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Catalog No. | RP01456 |
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Description | Recombinant Human GSTA1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Phe222) of human GSTA1 (Accession #NP_665683.1) fused with a 6×His tag at the C-terminus. |
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Purity | > 90% by SDS-PAGE. |
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Endotoxin | < 1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, mannitol and 0.01% Tween80. |
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Species | Human |
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Calculated MW | 27.1 kDa |
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Background
GSTA1 (Glutathione S-Transferase Alpha 1) is a Protein Coding gene. This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds. Glutathione S-transferases (GSTs) are involved in the detoxification of carcinogens and may be linked to carcinogenesis. As a vital component of GSTs, GSTA1 plays an important role in carcinogenesis. GSTA1 expression may be a target molecule in the early diagnosis and treatment of lung cancer. Human colonic adenocarcinoma (Caco-2) cells in culture undergo spontaneous differentiation into mature enterocytes in association with progressive increases in expression of glutathione S-transferase alpha-1 (GSTA1). GSTA1 levels may play a role in modulating enterocyte proliferation but do not influence differentiation or apoptosis. GSTA1 may play a key role during pregnancy.
Product information
Synonym | GSTA1; GST-epsilon; GST2; GSTA1-1; GTH1 |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.