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GSTA1 Rabbit pAb (A18266)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GSTA1 Rabbit pAb (A18266)

Western blot analysis of various lysates using GSTA1 Rabbit pAb (A18266) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GSTA1 Rabbit pAb (A18266)

Immunofluorescence analysis of C6 cells using GSTA1 Rabbit pAb (A18266) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GSTA1 Rabbit pAb (A18266)

Immunofluorescence analysis of L929 cells using GSTA1 Rabbit pAb (A18266) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GSTA1 Rabbit pAb (A18266)

Immunofluorescence analysis of U-2 OS cells using GSTA1 Rabbit pAb (A18266) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGSTA1 Rabbit pAb
Catalog No.A18266
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human GSTA1 (NP_665683.1).
SequenceMAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF
Gene ID
Swiss Prot
SynonymsGST2; GTH1; GSTA1-1; GST-epsilon; GSTA1
Calculated MW26kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, Mouse kidney, Mouse stomach, Rat liver, Rat kidney
Cellular locationCytoplasm
Customer validation

WB(Arabidopsis thaliana)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - GSTA1 Rabbit pAb (A18266)}

    Western blot - GSTA1 Rabbit pAb (A18266)

    Western blot analysis of various lysates using GSTA1 Rabbit pAb (A18266) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - GSTA1 Rabbit pAb (A18266)}

    Immunofluorescence - GSTA1 Rabbit pAb (A18266)

    Immunofluorescence analysis of C6 cells using GSTA1 Rabbit pAb (A18266) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GSTA1 Rabbit pAb (A18266)}

    Immunofluorescence - GSTA1 Rabbit pAb (A18266)

    Immunofluorescence analysis of L929 cells using GSTA1 Rabbit pAb (A18266) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GSTA1 Rabbit pAb (A18266)}

    Immunofluorescence - GSTA1 Rabbit pAb (A18266)

    Immunofluorescence analysis of U-2 OS cells using GSTA1 Rabbit pAb (A18266) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18266? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    재조합 단백질 (1)

    Secondary Antibodies (26)