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HRP-conjugated Goat anti-Rabbit IgG Heavy Chain (AS063)

Publications (52) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG Heavy Chain (AS063)

Western blot analysis of lysates from Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063) at 1:5000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameHRP-conjugated Goat anti-Rabbit IgG Heavy Chain
Catalog No.AS063
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW55kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000-10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRabbit IgG, Mouse IgG
Cellular location
Customer validation

WB(Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus, Triticum aestivum, Oryctolagus cuniculus, Mus musculus, Xenopus laevis, Other)

IHC(Mus musculus, Mus musculus )

IF(Mus musculus)

IHC(Mus musculus)

Co-IP(Xenopus laevis)

ChIP(Homo sapiens)

Co-IP(Homo sapiens)

FC(Homo sapiens)

IF(Homo sapiens)

RT-qPCR(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG Heavy Chain (AS063)}

    Western blot - HRP-conjugated Goat anti-Rabbit IgG Heavy Chain (AS063)

    Western blot analysis of lysates from Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (AS063) at 1:5000 dilution.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS063? Please let us know so that we can cite the reference in this datasheet.