| Product name | Recombinant Human IFN-alpha 1/13(Q114A) Protein |
|---|---|
| Catalog No. | RP00011 |
| Description | Recombinant Human IFN-alpha 1/13(Q114A) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Cys24-Glu189 (Ala114)) of human IFNA1 (Accession #NP_076918.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus. |
| Bio-Activity | Measured by its ability to stimulate GBP2 expression in 293T human embryonic kidney cells. 0.1-1 ng/mL of Recombinant Human IFNA1 can effectively Stimulating GBP2 expression. 2.Measured by its ability to stimulate human colon adenocarcinoma cells (Caco-2 cells). 10 ng/μL of Recombinant Human IFN-alpha 1/13(Q114A) can upregulate the expression of MDA5. |
| Purity | > 97% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 21.01 kDa |
| Synonym | IFNA1; IFL; IFN; IFN-ALPHA; IFN-alphaD; IFNA13; IFNA |
|---|---|
| Expression Host | E. coli |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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