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Recombinant Human IGF-I Protein (RP01608)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human IGF-I Protein
Catalog No.RP01608
DescriptionRecombinant Human IGF-I Protein is produced by Pichia expression system. The target protein is expressed with sequence (Gly49-Ala118) of human IGF-I (Accession #NP_001104755.1) fused with no additional amino acid.
Bio-ActivityMeasured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 53.5-214.2 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesHuman
Calculated MW7.65 kDa
SynonymIGF-1; IGF-I; IGF1A; IGFI; IGF?I; MGF; IGF1
Expression HostPichia
TagNo tag
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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