제품 > 항체 > 다중클론항체(pAb)

IGF1 Rabbit pAb (A11985)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using IGF1 Rabbit pAb (A11985) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IGF1 Rabbit pAb (A11985)

Immunohistochemistry analysis of paraffin-embedded Rat lung using IGF1 Rabbit pAb (A11985) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using IGF1 Rabbit pAb (A11985) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	IGF1 Rabbit pAb
Catalog No.	A11985
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IGF1 (NP_000609.1).
Sequence	PETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSARSVRAQRHTDMPKTQKEVHLKNASRGSAGNKN
Gene ID
Swiss Prot
Synonyms	IGF; MGF; IGFI; IGF-I; IGF1
Calculated MW	22kDa
Observed MW	18kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1500 - 1:3000 IHC-P 1:50 - 1:100 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	U-87MG, SKOV3, HeLa, Mouse liver, Mouse testis, Rat testis
Cellular location	Secreted.
Customer validation	WB(Mus musculus, Sus scrofa, Homo sapiens, Ctenopharyngodon idellus, Pampus argenteus, Oryctolagus cuniculus, Mus musculus, Other, Neovison vison) IF(Mus musculus)

Documents

Certificate of Compliance

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Research Area

IGF1 Rabbit pAb images

Western blot - IGF1 Rabbit pAb (A11985)

Immunohistochemistry - IGF1 Rabbit pAb (A11985)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Cell Res

Application:

IF:

25.617

Species:

Mus musculus

PMID:

32839552

Title:

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Journal of Agricultural and Food Chemistry

Application:

IF:

5.279

Species:

Sus scrofa

PMID:

32252520

Title:

An Early Fecal Microbiota Transfer Improves the Intestinal Conditions on Microflora and Immunoglobulin and Antimicrobial Peptides in Piglets

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

33398375

Title:

miR-613 inhibits the proliferation of human ovarian granulosa cells by arresting cell cycle progression via the targeting of IGF-1

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

IF:

6.08

Species:

Mus musculus

PMID:

36274835

Title:

Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischemia-reperfusion injury via intrinsic and paracrine mechanisms in a rat model - PMC

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

The British journal of nutrition

Application:

IF:

4.12

Species:

Ctenopharyngodon idellus

PMID:

35508921

Title:

Role of creatine supplementation on the myofiber characteristics and muscle protein synthesis of grass carp ( Ctenopharyngodon idellus )

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Acta pharmacologica Sinica

Application:

IF:

7.16

Species:

Homo sapiens

PMID:

36411339

Title:

Targeting PI3Kα overcomes resistance to KRasG12C inhibitors mediated by activation of EGFR and/or IGF1R

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Biology of reproduction

Application:

IF:

4.16

Species:

Pampus argenteus

PMID:

37228017

Title:

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Oryctolagus cuniculus

PMID:

37958699

Title:

lncRNA MSTRG4710 Promotes the Proliferation and Differentiation of Preadipocytes through miR-29b-3p/IGF1 Axis - PMC

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Oryctolagus cuniculus

PMID:

37958699

Title:

lncRNA MSTRG4710 Promotes the Proliferation and Differentiation of Preadipocytes through miR-29b-3p/IGF1 Axis - PMC

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens, Mus musculus

PMID:

36581638

Title:

Coal dust nanoparticles induced pulmonary fibrosis by promoting inflammation and epithelial-mesenchymal transition via the NF-B/NLRP3 pathway driven by IGF1/ROS-mediated AKT/GSK3 signals - PMC

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Brain Res Bull

Application:

WB, IF

IF:

3.715

Species:

Mus musculus

PMID:

34333050

Title:

Chemotherapy-induced peripheral neuropathy is promoted by enhanced spinal insulin-like growth factor-1 levels via astrocyte-dependent mechanisms

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Brain research

Application:

IF:

3.61

Species:

Mus musculus

PMID:

27328426

Title:

Hyperforin promotes post-stroke functional recovery through interleukin (IL) 17A-mediated angiogenesis

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Investigative ophthalmology & visual science

Application:

IF:

4.92

Species:

Mus musculus

PMID:

37145604

Title:

Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

6.54

Species:

Mus musculus

PMID:

35401920

Title:

Painful Diabetic Neuropathy Is Associated with Compromised Microglial IGF-1 Signaling Which Can Be Rescued by Green Tea Polyphenol EGCG in Mice

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

BMC genomics

Application:

IF:

4.4

Species:

Other

PMID:

39217297

Title:

Transcriptome analysis reveals high concentration of resveratrol promotes lipid synthesis and induces apoptosis in Siberian sturgeon (Acipenser baerii) - PMC

References for IGF1 Rabbit pAb (A11985)

Product:

IGF1 Rabbit pAb

Journal:

Life Sciences

Application:

IF:

6.1

Species:

Neovison vison

PMID:

32304761

Title:

Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway