Recombinant Human IL-17RA/CD217 Protein (RP00273)

Overview

Product name	Recombinant Human IL-17RA/CD217 Protein
Catalog No.	RP00273
Description	Recombinant Human IL-17RA/CD217 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu33-Trp320) of human IL17RA (Accession #NP_055154.3) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its ability to inhibit IL-17A induced IL-6 secretion by Hela cells. The ED₅₀ for this effect is 0.02 0.1 μg/mL in the presence of 10 ng/mL recombinant human IL 17A. 2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant human IL17RA , His Tag at 2 μg/mL (100 μL/well) can bind Recombinant human IL-17A, His Tag with a linear range of 31.25-65 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	34.29 kDa

Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. This transmembrane protein (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure.

Product information

Synonym	CANDF5; CD217; CDw217; IL-17RA; IL17R; IMD51; hIL-17R; IL17RA; CD217; CDw217; IL-17RA; IL17R; IMD51; hIL-17R
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.