| Product name | Recombinant Human IL-36 gamma/IL-1F9 Protein |
|---|---|
| Catalog No. | RP00035 |
| Description | Recombinant Human IL-36 gamma/IL-1F9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser18-Asp169) of human Interleukin-36 gamma (Accession #NP_062564.1 ). |
| Bio-Activity | 1.The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-36 gamma protein. After 7 hours, 2.5 ng/mL of human IL-36 gamma can effectively activate the NF-κB signaling. 2.Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for for this effect is 101.34-405.36 ng/mL. |
| Purity | > 92% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 17.03 kDa |
| Synonym | IL36G; IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1F9; IL1H1; IL1RP2 |
|---|---|
| Expression Host | E. coli |
| Tag | No tag |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
