Recombinant Human LFA-3/CD58 Protein (RP00257)

Product name	Recombinant Human LFA-3/CD58 Protein
Catalog No.	RP00257
Description	Recombinant Human LFA-3/CD58 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe 29 - Arg 215 ) of human CD58 (Accession #BC005930) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA.Immobilized Human CD2 at 2 μg/mL (100 μL/well) can bind Human CD58 with a linear range of 1.22-34.4 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized CD58 (LFA-3) Monoclonal Antibody at 1 μg/mL (25 μL/well) can bind Recombinant Human CD58 with a linear range of 0.5-6.4 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	48.25 kDa

Synonym	CD58; LFA-3; LFA3; ag3; CD58 molecule; LFA-3; LFA3; ag3
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.