Overview
| Product name | Recombinant Human Leptin receptor/LEP-R/CD295 Protein |
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| Catalog No. | RP01248 |
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| Description | Recombinant Human Leptin receptor/LEP-R/CD295 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr20-Asp839) of human Leptin R/CD295 (Accession #NP_002294.2) fused with a Fc, 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 protein，the EC50 of Leptin R/CD295 is 261.45 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM MES, 100mM NaCl, 1mM DTT, pH6.5.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 120.46 kDa |
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Product information
| Synonym | CD295; LEP-R; LEPRD; OB-R; OBR; LEPR |
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| Expression Host | HEK293 cells |
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| Tag | C-hFc&His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
