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Leptin Receptor Rabbit mAb (A19076)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Leptin Receptor Rabbit mAb (A19076)

Western blot analysis of various lysates using Leptin Receptor Rabbit mAb (A19076) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min .

ABclonal:Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Leptin Receptor Rabbit mAb (A19076) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Leptin Receptor Rabbit mAb (A19076) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Leptin Receptor Rabbit mAb (A19076) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameLeptin Receptor Rabbit mAb
Catalog No.A19076
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0454
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Leptin Receptor (P48357).
SequenceEEQGLINSSVTKCFSSKNSPLKDSFSNSSWEIEAQAFFILSDQHPNIISPHLTFSEGLDELLKLEGNFPEENNDKKSIYYLGVTSIKKRESGVLLTDKSRV
Gene ID
Swiss Prot
SynonymsOBR; OB-R; CD295; LEP-R; LEPRD; Leptin Receptor
Calculated MW132kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHep G2, Mouse heart, Mouse lung, Rat liver
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.
Customer validation

WB(Mus musculus, Mus musculus )

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Leptin Receptor Rabbit mAb (A19076)}

Western blot - Leptin Receptor Rabbit mAb (A19076)

Western blot analysis of various lysates using Leptin Receptor Rabbit mAb (A19076) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min .
ABclonal:Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)}

Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Leptin Receptor Rabbit mAb (A19076) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)}

Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Leptin Receptor Rabbit mAb (A19076) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)}

Immunohistochemistry - Leptin Receptor Rabbit mAb (A19076)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Leptin Receptor Rabbit mAb (A19076) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19076? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

재조합 단백질 (1)

Secondary Antibodies (26)