Overview
| Product name | Recombinant Human Neurogranin/NRGN Protein |
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| Catalog No. | RP01814 |
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| Description | Recombinant Human Neurogranin/NRGN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp2-Asp78) of human NRGN/Neurogranin (Accession #) fused with hFc tag at the C-terminus. |
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | < 0.01EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM Tris 150mM Nacl, pH 7.5. |
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| Species | Human |
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| Calculated MW | 33.45 kDa |
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Background
Neurogranin is as a small protein encoded by the NRGN gene. it is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability. It is a potential biomarker of neurological and mental diseases.
Product information
| Synonym | RC3; hng; NRGN; Neurogranin |
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| Expression Host | HEK293 cells |
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| Tag | C-hFC |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
