Overview
| Product name | Recombinant Human Orexin-A/Hypocretin/HCRT Protein |
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| Catalog No. | RP01920 |
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| Description | Recombinant Human Orexin-A/Hypocretin/HCRT Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln34-Leu66) of Human Orexin A/Hypocretin-1 (Accession #NP_001515.1) fused with a hFc tag at the N-terminus. |
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| Purity | > 90% by SDS-PAGE. |
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| Endotoxin | < 0.1EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 29.54 kDa |
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Background
Orexin A/Hypocretin-1, Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggestedBy Similarit，Orexin-A，Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity .Stimulates food intake.Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.
Product information
| Synonym | HCRT; OX; PPORX; PPOX; Hypocretin neuropeptide precursor; Hypocretin; Hcrt; Orexin precursor; Prepro-orexin; Preprohypocretin; Cleaved into: Orexin-A; Hypocretin-1; Hcrt1; Orexin-B; Hypocretin-2; Hcrt2 |
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| Expression Host | HEK293 cells |
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| Tag | N-hFc |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
