제품 > 항체 > 단일클론항체(mAb)

Orexin B Rabbit mAb (A23383)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of wild type (WT) and 293F cells transfected with Orexin B, using Orexin B Rabbit mAb (A23383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Orexin B Rabbit mAb (A23383)

Immunofluorescence analysis of Orexin B-293F transfected and control 293F cells using Orexin B Rabbit mAb (A23383) at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Overview

Product name	Orexin B Rabbit mAb
Catalog No.	A23383
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60837

Background

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

Immunogen information

Immunogen	Recombinant protein of human Orexin B/Hypocretin-2
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	OX; PPOX; NRCLP1; Orexin B
Calculated MW	13kDa
Observed MW	32kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293F transfected with Orexin B
Cellular location	Cell junction, Cytoplasmic vesicle, Rough endoplasmic reticulum, synapse

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구내분비 & 대사내분비 및 대사 질환비만

Orexin B Rabbit mAb images

Western blot - Orexin B Rabbit mAb (A23383)

Immunofluorescence - Orexin B Rabbit mAb (A23383)

Immunofluorescence analysis of Orexin B-293F transfected and control 293F cells using Orexin B Rabbit mAb (A23383) at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.