Product name | Recombinant Human Siglec-2/CD22 Protein |
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Catalog No. | RP01199 |
Description | Recombinant Human Siglec-2/CD22 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp20-Arg687) of human Siglec-2/CD22 (Accession #NP_001762.2) fused with a Fc, 6×His tag at the C-terminus. |
Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD22 (Catalog: RP01199) at 0.5 μg/mL (100 μL/well) can bind anti CD22 Rabbit mAb with a linear range of 0.01-1.28ng/mL. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Calculated MW | 101.93 kDa |
Synonym | CD22; SIGLEC-2; SIGLEC2; CD22 molecule; SIGLEC-2; SIGLEC2 |
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Expression Host | HEK293 cells |
Tag | C-hFc&His |
Swiss-Prot | |
Gene ID |
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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