Recombinant Human Siglec-3/CD33 Protein (RP00159)

Overview

Product name	Recombinant Human Siglec-3/CD33 Protein
Catalog No.	RP00159
Description	Recombinant Human Siglec-3/CD33 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp18-His259) of human CD33/Siglec-3 (Accession #NP_001763.3) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human CD33 Protein at 1μg/mL (100 μL/well) can bind CD33 Rabbit pAb with a linear range of 0.03-1.66 ng/mL.
Purity	> 97% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	53.63 kDa

Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.

Product information

Synonym	CD33; SIGLEC-3; SIGLEC3; p67; CD33 molecule; SIGLEC-3; SIGLEC3; p67
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.