Overview
Product name | Recombinant Mouse CXCL1/GRO-alpha Protein |
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Catalog No. | RP01615 |
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Description | Recombinant Mouse CXCL1/GRO-alpha Protein is produced by Pichia expression system. The target protein is expressed with sequence (Arg20-Lys96) of mouse CXCL1/GRO-alpha (Accession #NP_032202.1) fused with no additional amino acid. |
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Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse CXCL1 at 2μg/mL (100 μL/well) can bind GRO alpha Rabbit pAb with a linear range of 0.6-2.37 ng/mL.
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Purity | > 95% by SDS-PAGE. |
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Endotoxin | <0.1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Mouse |
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Calculated MW | 9.31 kDa |
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Background
This antimicrobial protein belongs a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4.
Product information
Synonym | FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1; CXCL1 |
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Expression Host | Pichia |
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Tag | No tag |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.