Recombinant Mouse IL-15RA/CD215 Protein (RP01373)

Overview

Product name	Recombinant Mouse IL-15RA/CD215 Protein
Catalog No.	RP01373
Description	Recombinant Mouse IL-15RA/CD215 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly33-Lys205) of mouse IL15RA/CD215 (Accession #NP_032384.1) fused with a Fc, 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA.Immobilized Human IL15 at 1 μg/mL (100 μL/well) can bind Mouse IL15RA with a linear range of 0.3-77 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	<0.1EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Mouse
Calculated MW	45.16 kDa

Mouse interleukin-15 receptor subunit alpha, also known as Il15ra, is a high-affinity receptor for interleukin-15.Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits (Common gamma chain, orgamma c) to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cellspresents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide varietyof Tand B cells and non-lymphoid cells. Human Il15ra shares 45% amino acid sequence homology with themouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified, resulting fromalternative splicing events involving different exons.

Product information

Synonym	IL-15RA; CD215; AA690181; IL15RA
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.