| Product name | Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein |
|---|---|
| Catalog No. | RP01395 |
| Description | Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val23-Gly258) of mouse TNFR2/CD120b/TNFRSF1B (Accession #NP_035740.2) fused with a hFc tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-α at 2 μg/mL (100 μL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL. 2.Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of Recombinant Mouse TNFα. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Mouse |
| Calculated MW | 51.29 kDa |
| Synonym | TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; TNFRSF1B; TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; TNFRSF1B |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-hFc |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
