Overview
Product name | Recombinant Mouse TNFRSF9/4-1BB/CD137 Protein |
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Catalog No. | RP01560 |
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Description | Recombinant Mouse TNFRSF9/4-1BB/CD137 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Leu211) of mouse TNFRSF9/4-1BB/CD137 (Accession #NP_001070976.1) fused with a hFc tag at the C-terminus. |
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Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFSF9(Catalog: RP00766) at 5μg/mL (100 μL/well) can bind Mouse TNFRSF9/4-1BB/CD137
(Catalog: RP01560) with a linear range of 0.005-1.41 ng/mL.
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Purity | > 97% by SDS-PAGE. |
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Endotoxin | <1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Mouse |
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Calculated MW | 46.03 kDa |
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Background
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses.
Product information
Synonym | 4-1BB ; CD137 ; CDw137 ; ILA; TNFRSF9; 4-1BB ; CD137 ; CDw137 ; ILA; TNFRSF9 |
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Expression Host | HEK293 cells |
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Tag | C-hFc |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.