Recombinant Mouse VCAM-1/CD106 Protein (RP01176)

Product name	Recombinant Mouse VCAM-1/CD106 Protein
Catalog No.	RP01176
Description	Recombinant Mouse VCAM-1/CD106 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu698) of mouse VCAM-1/CD106 (Accession #NP_035823.3) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/mL with 100 μL/well), approximately 70%-80% cells will adhere after 1 hour at 37℃.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Mouse
Calculated MW	77.73 kDa

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.