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ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

Flow cytometry: 1X10^6 RAW 264.7 cells (negative control, left) and MS1 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

Flow cytometry: 1X10^6 MS1 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb
Catalog No.A25748
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65686-ABflo488
Predicted to enable integrin binding activity and primary amine oxidase activity. Acts upstream of or within several processes, including cellular response to glucose stimulus; chorio-allantoic fusion; and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Located in apical plasma membrane. Is expressed in several structures, including brain; embryo mesenchyme; extraembryonic component; heart; and hemolymphoid system. Human ortholog(s) of this gene implicated in hypertension. Orthologous to human VCAM1 (vascular cell adhesion molecule 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-310 of mouse VCAM-1/CD106 (NP_035823.3).
SequenceFKIEISPEYKTIAQIGDSMALTCSTTGCESPLFSWRTQIDSPLNAKVRTEGSKSVLTMEPVSFENEHSYLCTATCGSGKLERSIHVDIYSFPKDPEIQFSGPLEVGKPVTVKCLAPDIYPVYRLEIDLFKGDQLMNRQEFSSEEMTKSLETKSLEVTFTPVIEDIGKALVCRAKLHIDQIDSTLKERETVKELQVYISPRNTTISVHPSTRLQEGGAVTMTCSSEGLPAPEIFWGRKLDNEVLQLLSGNATLTLIAMRMEDSGVYVCEGVNLIGRDKAEVELVVQE
Gene ID
Swiss Prot
SynonymsCD106; Vcam-1
Calculated MW38kDa/81kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein,Secreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

    Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

    Flow cytometry: 1X10^6 RAW 264.7 cells (negative control, left) and MS1 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748)

    Flow cytometry: 1X10^6 MS1 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse VCAM-1/CD106 mAb (A25748, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

    ELISA 키트 (1)

    재조합 단백질 (2)

    항체 (3)

    Secondary Antibodies (26)