ABflo™ 는 성능 면에서 기존 마우스 단일클론 항체를 능가하는 새로운 유세포분석용 재조합 토끼 단일클론 항체입니다.
선별된 클론 & 뛰어난 성능
SDS-PAGE
Results : Recombinant human CD31 protein was detected using SDS-PAGE and Coomassie
Brilliant Blue staining, showing a distinct target band in the 85-105 kDa range, with a protein purity greater than 95%.
Bioactivity-ELISA
Results: Mouse anti-human CD31 antibody (1 µg/mL, 25 µL/well) was able to bind to human CD31 protein, with a linear range of 24.34-29.48 ng/mL
HEL cells
Results: Antibodies obtained from immunization with CD31 active protein showed significantly improved signal transition in flow cytometry under the same experimental conditions compare with competitors.
Stable cell line: immunogen
Results: Flow cytometry analysis of stable transfected cell lines demonstrated a signal transition that was 2.4 orders of magnitude higher compare with control cells, indicating the successful generation of stable transfected cell lines.
CHO Claudin18.2 stable cell line
Results: Antibodies obtained from Claudin 18.2 stable transfected cell line immunization showed significantly improved signal transition in flow cytometry under the same experimental conditions compared with competitors.
U937 cells (negative control)
Jurkat cells
The results showed that under the same experimental conditions, ABclonal antibody exhibited no significant signal transition in the negative control sample (U937 cells) but displayed a noticeable signal transition in the positive sample (Jurkat cells), indicating higher antibody specificity compared to competitors.
CTLL-2 cells
MOLT-4 cells
The results showed that under the same experimental conditions, ABclonal antibody exhibited significant signal transition, even with lower usage compared to competitor antibodies.
C2C12 cells (negative control)
CTLL-2 cells
Results showed that under the same experimental conditions, the clone ARC54525-ABf647 from ABclonal outperformed the clone ARC54526-ABf647 and competitors, leading to its selection for production.
PC-3 cells ( negative control )
PBMC
C2C12 cells (low expression)
bEnd.3 cells
포괄적인 검증 & 엄격한 데이터
293F cells (negative control)
MOLT-4 cells
Human PBMC
C57BL/6 mouse Splenocytes
PC-3 cells
C57BL/6 mouse Splenocytes
Jurkat cells
Jurkat cells
A20 cells
좋은 호환성 & 높은 재현성
The results demonstrate that when utilizing ABflo™ 647-conjugated antibodies targeting human CD79b in comparison to competitor antibodies conjugated with APC, under identical experimental conditions, detection was conducted using the APC channel on various flow cytometers, including Beckman cytoflex™ S, Agilent NovoCyte™, and FongCyte™. ABclonal flow cytometry antibodies consistently exhibited comparable signal transitions across all three devices, outperforming the competitors with significantly enhanced signal transitions.
안정적인 공급망 & 보장된 가용성
안정적인 공급망을 통해 시기 적절하고 편리한, 빠르고 안전한 신뢰할 수 있는 배송을 보장합니다. 당사의 공급 시스템은 유연성을 제공하여 고객의 특정 요구 사항을 충족하도록 기존 카탈로그 제품의 맞춤형 생산 및 라벨링을 제공할 수 있습니다.
