ABScript III One Step RT-qPCR Probe Kit with UDG V5 (RK20412)

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. The heat-liable UDG in this product could degrade U-contained contamination in room temperature, and inactivated in 50℃, which could prevent false positive results without affect the efficiency and sensitivity. The ABScript III Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript III Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5'-3' exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript III One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample

One Step probe RT-qPCR，RNA viruses detection

Upon receipt, store all components at -20°C.