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[KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Publications (46) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse,Rat

ABclonal:[KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb
ABclonal:Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Western blot analysis of lysates from Raw264.7 cells, using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Western blot analysis of lysates from HepG2 cells, using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Western blot analysis of lysates from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockdown (KD) HeLa cells, using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunofluorescence analysis of paraffin-embedded human spleen using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb
Catalog No.A19062
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53508
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human Heme Oxygenase 1 (HO-1/HMOX1) (NP_002124.1).
SequenceMERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQA
Gene ID
Swiss Prot
SynonymsHO-1; HSP32; HMOX1D; bK286B10
Calculated MW33kDa
Observed MW33kDa
ReactivityHuman, Mouse,Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Hep G2, RAW 264.7
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein.
Customer validation

WB(Rattus norvegicus, Mus musculus, Mus musculus , Homo sapiens, Mus musculusRattus norvegicus, Mus musculus, Ovis aries, Other)

IHC(Rattus norvegicus, Mus musculus, Homo sapiens)

IHC(Homo sapiens, Mus musculus)

Co-IP(Mus musculus )

RT-qPCR(Homo sapiens)

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Western blot analysis of lysates from Raw264.7 cells, using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Western blot analysis of lysates from HepG2 cells, using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Western blot - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Western blot analysis of lysates from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockdown (KD) HeLa cells, using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Immunohistochemistry - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)}

Immunofluorescence - [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062)

Immunofluorescence analysis of paraffin-embedded human spleen using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)