Description
This product is a blue-colored universal SYBR Green reagent, convenient for sampling and using specially designed reference dye (ROX) for higher sensitivity and resolution. It is compatible with all models of fluorescence quantitative PCR instruments (including High ROX, Low ROX, and No ROX required instruments). The reagent introduces a dUTP/UDG system preventing the impact of amplification product contamination on qPCR and ensuring the accuracy of the results. UDG can quickly degrade pollutants containing U at room temperature, and UDG quickly deactivates when pre denatured at 95 ℃, without affecting the efficiency and sensitivity of qPCR. Hot start Taq DNA polymerase is used for amplification, which enhances the specificity of the product. Overall, this product provides a reliable and versatile solution for SYBR Green-based qPCR experiments.
Product Features
- Extremely sensitive: This product adopts a brand new mixed enzyme formula that provides higher sensitivity, making it especially suitable for difficult samples and low-abundance gene detection;
- Pollution prevention: The dUTP-UDG pollution prevention system reduces the risk of aerosol pollution, making it safer to handle;
- Stability: The optimized buffer formula provides better reagent stability, with no significant impact on performance when stored at 37℃ for one week. It can also be transported in ice bags for a short time without compromising its quality;
- High usability: The product is premixed with universal reference dyes (ROX), eliminating the need to add ROX separately. This makes it suitable for use on various instrument platforms, enhancing its usability.
- Convenient: This product is a blue-colored universal SYBR Green reagent, convenient for sampling.
Applications
Dye-based qPCR
Storage
This product should be stored at -20℃ for long-term storage and should be protected from light.
Product Components
| Components | Component number | Size-1
1 mL | Size-2
5 mL |
| BrightCycle Blue Universal SYBR Green qPCR Mix with UDG * | RM21225 | 1 mL | 5 × 1 mL |
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