Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein (RP00066)

Overview

Product name	Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein
Catalog No.	RP00066
Description	Recombinant Human Fc-gamma RIII alpha/FCGR3A/CD16a Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gly17-Gln208) of human Fc gamma RIIIA/CD16a (Accession #NP_001121065.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Human FCGR3A at 1 μg/mL (100 μL/well) can bind FCGR3A Mouse mAb with a linear range of 0.13-6.1 ng/mL. 2.Measured by its binding ability in a functional ELISA.Immobilized Human FCGR3A at 1 μg/mL (100 μL/well) can bind FCGR3A Mouse mAb with a linear range of 0.06-7.1 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	22.68 kDa

This protein is a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. The protein is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia.

Product information

Synonym	CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; FCGR3A; CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; Fc fragment of IgG receptor IIIa
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.