Overview
Product name | Recombinant Human Fc-gamma RIII alpha/FCGR3A /CD16a Protein |
---|
Catalog No. | RP01043 |
---|
Description | Recombinant Human Fc-gamma RIII alpha/FCGR3A /CD16a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly17-Gln208) of human Fc gamma RIIIA/CD16a (Accession #NP_001121065.1) fused with an Fc, 6×His tag at the C-terminus. |
---|
Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human FCGR3A at 1 μg/mL (100 μL/well) can bind FCGR3A Mouse mAb with a linear range of 0.13-7.9 ng/mL.
|
---|
Purity | > 95% by SDS-PAGE. |
---|
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
---|
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
---|
Species | Human |
---|
Calculated MW | 48.62 kDa |
---|
Background
This protein is a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. The protein is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia.
Product information
Synonym | CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; FCGR3A; CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; Fc fragment of IgG receptor IIIa |
---|
Expression Host | HEK293 cells |
---|
Tag | C-hFc&His |
---|
Swiss-Prot | |
---|
Gene ID | |
---|
Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
---|
Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
---|
* For research use only. Not for therapeutic or diagnostic purposes.