Description
TruePol 2X PCR Mix for NGS is a premixed solution derived from polymerase of the B family. It is easy to use, containing DNA polymerase, buffer, Mg2+, and dNTPs. For amplification, simply add DNA template, primers, and water to the system for amplification. This polymerase comes from a unique polymerase mutation library, offering strong extension capabilities that improve yield and specificity without significant GC bias. It has 5'-3' continuous synthesis activity and 3'-5' exonuclease activity, resulting in blunt-end amplification products.
All batches of TruePol Mix undergo rigorous quality control to ensure the consistent performance of each batch of reagent.
Product Features
- Convenient and Reliable: The ready-to-use premixed reagent ensures a simple process, guaranteeing accuracy and reproducibility.
- Compatible with Multiple Library Types: It is compatible with regular DNA libraries, as well as micro-volume sample libraries such as cfDNA, FFPE DNA, ChIP DNA, and transcriptome libraries.
- High Stability: The enzyme performance is not significantly affected after being stored at 4°C, 25°C, or 37°C for two weeks, or after 50 freeze-thaw cycles.
Applications
High-throughput amplification in library construction.
Storage
All components should be stored at -20°C.
Product Components
| Components | 24 RXN | 96 RXN |
| 2X PCR Mix | 600 μL | 1.2 mL × 2 |
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