Description
MethyLight qPCR Probe Set is designed for probe-based qPCR reactions. It is a powerful tool for MSP analysis of methylated DNA and multi-target detection in epigenetic research. Through buffer optimization, it can be used for multiple MSP (methylation specific PCR) analysis on ssDNA containing uracil through DNA methylation by bisulfite or enzyme conversion. MethyLight qPCR Probe Set containing antibody-mediated hot start DNA polymerase, dNTPs, MgCl2, KCI and other stabilizers, improves molecular recognition ability and sensitively identifies methylated and non-methylated sequences. It helps user efficiently obtain accurate quantification and good repeatability.
This product contains antibody-mediated MethyLight HS Taq Polymerase, MethyLight 5X qPCR Reaction Buffer (No dNTPs and Mg2+), dNTPs, and MgCl2 required for MethyLight qPCR reactions except primers, probes and templates.
Product Features
- Specificity: Specificly identify methylated and non-methylated templates;
- Universality: Compatible with DNA enzymatic conversion and bisulfite conversion;
- Stability: Can be stored for 40d at 37°C;
Storage
Upon receipt, store all components at -20°C.
Product Components
| Components | Component number | Size-1
100 RXN(20 μL/RXN) | Size-2
500 RXN(20 μL/RXN) |
| MethyLight HS Taq Polymerase | RM21231 | 100μL | 500μL |
| MethyLight 5X qPCR Reaction Buffer (No dNTPs and Mg2+) | RM21233 | 400 μL | 1mL*2 |
| dNTPs (10 mM each) | RM20120 | 50 μL | 250 μL |
| MgCl2 (50 mM) | RM20144 | 1.25 mL | 1.25 mL |
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