제품 > 항체 > 단일클론항체(mAb)

ABflo® 700 Rat anti-Mouse CD3 mAb (A27432)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 700 Rat anti-Mouse CD3 mAb (A27432)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 700 Rat IgG2b isotype control (5 μl/Test, left) or ABflo® 700 Rat anti-Mouse CD3 mAb (A27432, 5 μl/Test, right).

Overview

Product nameABflo® 700 Rat anti-Mouse CD3 mAb
Catalog No.A27432
Host speciesRat
Purification methodAffinity purification
IsotypeIgG2b, κ
CloneNo.ATC0031-ABflo700
Predicted to enable identical protein binding activity and transmembrane signaling receptor activity. Involved in positive thymic T cell selection. Part of alpha-beta T cell receptor complex. Is expressed in thymus primordium. Human ortholog(s) of this gene implicated in immunodeficiency 19 and severe combined immunodeficiency. Orthologous to human CD3D (CD3d molecule). Predicted to enable several functions, including SH3 domain binding activity; identical protein binding activity; and protein heterodimerization activity. Involved in nervous system development and positive regulation of cell adhesion. Acts upstream of or within several processes, including positive regulation of T cell activation; positive regulation of cytokine production; and regulation of signal transduction. Located in several cellular components, including dendritic spine; external side of plasma membrane; and immunological synapse. Part of alpha-beta T cell receptor complex. Is expressed in colon and hemolymphoid system. Human ortholog(s) of this gene implicated in immunodeficiency 18. Orthologous to human CD3E (CD3e molecule).
ImmunogenA synthesized peptide derived from mouse CD3.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsT3d; CD3; T3e; CD3epsilon
Calculated MW19kDa/21kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Cell membrane.
    ABclonal:Flow CytoMetry - ABflo® 700 Rat anti-Mouse CD3 mAb (A27432)}

    Flow CytoMetry - ABflo® 700 Rat anti-Mouse CD3 mAb (A27432)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 700 Rat IgG2b isotype control (5 μl/Test, left) or ABflo® 700 Rat anti-Mouse CD3 mAb (A27432, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.