제품 > 항체 > 다중클론항체(pAb)

AMPKα1 Rabbit pAb (A21813)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - AMPKα1 Rabbit pAb (A21813)

Western blot analysis of lysates from Mouse liver, using AMPKα1 Rabbit pAb (A21813) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameAMPKα1 Rabbit pAb
Catalog No.A21813
Host species
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human AMPKα1 (NP_006242.5).
SequenceIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRL
Gene ID
Swiss Prot
SynonymsAMPK; AMPKa1; AMPK alpha 1; AMPKα1
Calculated MW64kDa
Observed MW64kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver
Cellular locationapical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus.
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - AMPKα1 Rabbit pAb (A21813)}

Western blot - AMPKα1 Rabbit pAb (A21813)

Western blot analysis of lysates from Mouse liver, using AMPKα1 Rabbit pAb (A21813) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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