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Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from C6 cells using Phospho-AMPKα1-S485 Rabbit pAb (AP0871) at 1:2000 dilution. C6 cells were treated by λpp(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product name	Phospho-AMPKα1-S485 Rabbit pAb
Catalog No.	AP0871
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S485 of human PRKAA1 (NP_006242.5).
Sequence	SGSVSN
Gene ID
Swiss Prot
Synonyms	AMPK; AMPKa1; AMPK alpha 1; Phospho-AMPKα1-S485
Calculated MW	64kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C6
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Arabidopsis thaliana, Other, Sus scrofa) IHC(Homo sapiens) IHC(Ctenopharyngodon idellus) WB(Ctenopharyngodon idellus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-AMPKα1-S485 Rabbit pAb images

Western blot - Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Cell Stress Chaperones

Application:

IF:

3.66

Species:

Mus musculus

PMID:

33743152

Title:

Heat stress promotes lipid accumulation by inhibiting the AMPK-PGC-1α signaling pathway in 3T3-L1 preadipocytes

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Rattus norvegicus

PMID:

34385063

Title:

Hexavalent chromium inhibits the formation of neutrophil extracellular traps and promotes the apoptosis of neutrophils via AMPK signaling pathway

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

Application:

IF:

3.99

Species:

Rattus norvegicus

PMID:

37918276

Title:

Mitigation of cisplatin-induced nephrotoxicity by chelidonic acid in Wistar rats

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

4.81

Species:

Homo sapiens

PMID:

37934902

Title:

Maackiain suppresses the development of cervical cancer via AMPK priming autophagy

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

37423515

Title:

Zanthoxylum bungeanum amides ameliorates nonalcoholic fatty liver via regulating gut microbiota and activating AMPK/Nrf2 signaling

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Diabetology & metabolic syndrome

Application:

IF:

5.39

Species:

Mus musculus

PMID:

37391779

Title:

The role of Huidouba in regulating skeletal muscle metabolic disorders in prediabetic mice through AMPK/PGC-1/PPAR pathway - PMC

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

37960350

Title:

1,25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function - PMC

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Cancers

Application:

WB, IHC

IF:

6.575

Species:

Homo sapiens

PMID:

32580279

Title:

COX5B-Mediated Bioenergetic Alteration Regulates Tumor Growth and Migration by Modulating AMPK-UHMK1-ERK Cascade in Hepatoma

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Application:

IF:

4.56

Species:

Arabidopsis thaliana

PMID:

38328791

Title:

The effect of inhibiting hindbrain A2 noradrenergic neurons by 6-Hydroxydopamine on lipopolysaccharide-treated male rats autistic animal model - PMC

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Mus musculus,Other

PMID:

38259150

Title:

Studying Antifatigue Mechanism of Tyr-Pro-Leu-Pro in Exercise Mice Using Label-Free Proteomics

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Gene

Application:

IF:

3.91

Species:

Homo sapiens

PMID:

37257791

Title:

TLR4 inhibited autophagy by modulating PI3K/AKT/mTOR signaling pathway in Gastric cancer cell lines

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

5.8

Species:

Sus scrofa

PMID:

38705537

Title:

EGCG alleviates heat-stress-induced fat deposition by targeting HSP70 through activation of AMPK-SIRT1-PGC-1 in porcine subcutaneous preadipocytes

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IHC, WB

IF:

Species:

Ctenopharyngodon idellus

PMID:

39218924

Title:

Methionine deficiency inhibited pyroptosis in primary hepatocytes of grass carp (Ctenopharyngodon idella): possibly via activating the ROS-AMPK-autophagy axis - PMC

References for Phospho-AMPKα1-S485 Rabbit pAb (AP0871)

Product:

Phospho-AMPKα1-S485 Rabbit pAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.2

Species:

Mus musculus

PMID:

39272560

Title:

Investigation of the Lipid-Lowering Activity and Mechanism of Three Extracts from Astragalus membranaceus, Hippophae rhamnoides L., and Taraxacum mongolicum Hand. Mazz Based on Network Pharmacology and In Vitro and In Vivo Experiments - PMC