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Phospho-SRC Family-Y416 Antibody kit (RK06002)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0480)
ABclonal:Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0480)
ABclonal:Western blot - Src Rabbit pAb (A18240)

Overview

Product namePhospho-SRC Family-Y416 Antibody kit
Catalog No.RK06002

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-SRC Family-Y416 Rabbit pAbAP0480WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionHuman, Mouse, Rat
Src Rabbit pAbA18240WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionHuman, Mouse, Rat
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 430-530 of human Src (NP_005408.1).
A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_005408.1).
SequenceKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQY
EDNEY
Gene ID2534/3055/3932/4067/6714/7525
Swiss protP06241/P08631/P06239/P07948/P12931/P07947
SynonymsASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416
Calculated MW59kDa/60kDa
Observed MW60kDa
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Cellular locationCell membrane,Cytoplasm,Mitochondrion inner membrane,Nucleus,cytoskeleton.
Phospho-SRC Family-Y416 Rabbit pAb (AP0480)
ABclonal:Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0480)}
Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0480)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0480). NIH/3T3 cells were treated by pervanadate.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0480)}
Western blot - Phospho-SRC Family-Y416 Rabbit pAb (AP0480)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb ( A18240). NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Src Rabbit pAb (A18240)
ABclonal:Western blot - Src Rabbit pAb (A18240)}
Western blot - Src Rabbit pAb (A18240)

Western blot analysis of various lysates using Src pAb (A18240) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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