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Phospho-SRC Family-Y416 Rabbit pAb (AP0480)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0480). NIH/3T3 cells were treated by pervanadate.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb ( A18240). NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-SRC Family-Y416 Rabbit pAb
Catalog No.	AP0480
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_005408.1).
Sequence	EDNEY
Gene ID
Swiss Prot
Synonyms	ASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416
Calculated MW	59kDa/60kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by PDGF or pervanadate
Cellular location	Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton.
Customer validation	WB(Homo sapiens)