Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from HepG2 cells, using Phospho-SRC Family-Y416 Rabbit mAb (AP1370) at 1:300 dilution. HepG2 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit mAb (AP1370) at 1:300 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from C6 cells using Phospho-SRC Family-Y416 Rabbit mAb (AP1370) at 1:1000 dilution. C6 cells were treated by Pervanadate (0.5 mM) at 37℃ for 40 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
| Product name | Phospho-SRC Family-Y416 Rabbit mAb |
|---|---|
| Catalog No. | AP1370 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC57736 |
| Immunogen | A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_005408.1). |
|---|---|
| Sequence | NEYTAR |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416 |
| Calculated MW | 60kDa |
| Observed MW | 62kDa/60kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HepG2 treated by TGFβ, NIH/3T3 treated by PDGF, C6 treated by Pervanadate |
| Cellular location | Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, Cytoskeleton. |
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