Reviews (3)Publications (7) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0452) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
| Product name | Phospho-SRC Family-Y416 Rabbit pAb |
|---|---|
| Catalog No. | AP0452 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_005408.1). |
|---|---|
| Sequence | NEYTA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416 |
| Calculated MW | 60kDa |
| Observed MW | 60kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | NIH/3T3 treated by PDGF |
| Cellular location | Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton. |
| Customer validation | (Human breast cancer, H460 and A549, Human lung cancer cell) WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Mus musculus, Rattus norvegicus) |
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