Biotinylated Recombinant Human B7-2/CD86 Protein (RP00561B)

Overview

Product name	Biotinylated Recombinant Human B7-2/CD86 Protein
Catalog No.	RP00561B
Description	Biotinylated Recombinant Human B7-2/CD86 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu26-Pro247) of Human B7-2/CD86 (Accession #P42081-1) fused with a C-His&Avi tag at the C-terminus.
Purity	> 95% by SDS-PAGE
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	28.2 kDa

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Product information

Synonym	41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.