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PE Rabbit anti-Human CD86 mAb (A26125)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD86 mAb (A26125)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Raji cells (right) were surface-stained with PE Rabbit anti-Human CD86 mAb (A26125, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD86 mAb (A26125)

Flow cytometry: 1X10^6 Raji cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD86 mAb (A26125, 5 μl/Test, right).

Overview

Product namePE Rabbit anti-Human CD86 mAb
Catalog No.A26125
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54012-PE
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-241 of human CD86 (NP_787058.5).
SequenceLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQP
Gene ID
Swiss Prot
SynonymsB70; B7-2; B7.2; LAB72; CD28LG2
Calculated MW38kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein.

Documents

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ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD86 mAb (A26125)}

Flow CytoMetry - PE Rabbit anti-Human CD86 mAb (A26125)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Raji cells (right) were surface-stained with PE Rabbit anti-Human CD86 mAb (A26125, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD86 mAb (A26125)}

Flow CytoMetry - PE Rabbit anti-Human CD86 mAb (A26125)

Flow cytometry: 1X10^6 Raji cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD86 mAb (A26125, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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