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Biotinylated Recombinant Human Mesothelin/MSLN Protein (RP02448B)

Datasheet

Overview

Product nameBiotinylated Recombinant Human Mesothelin/MSLN Protein
Catalog No.RP02448B
DescriptionBiotinylated Recombinant Human Mesothelin/MSLN Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Glu296-Gly580) of Human Mesothelin fused with a His tag and Avi tag at the C-terminal.
Purity> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
Endotoxin< 1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesHuman
Calculated MW35.2 kDa
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
SynonymMPF; SMRP; MSLN; SMRP
Expression HostHEK293 cells
TagC-His&Avi
Swiss-Prot
Gene ID
StorageStore at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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