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Recombinant Human Mesothelin/MSLN Protein (RP01220)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human Mesothelin/MSLN Protein
Catalog No.RP01220
DescriptionRecombinant Human Mesothelin/MSLN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu296-Gly580) of human Mesothelin (Accession #NP_005814.2) fused with a Fc, 6×His tag at the C-terminus.
Bio-ActivityLoaded Human Mesothelin/MSLN Protein, C-hFc&His (Catalog: RP01220) on ProA Biosensor, can bind Human CA125 Protein, C-His&Avi (Catalog: RP01417) with an affinity constant of 17.2 nM as determined in BLI assay (Gator).
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW59.02 kDa
SynonymMPF; SMRP; MSLN; SMRP
Expression HostHEK293 cells
TagC-hFc&His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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