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Mesothelin Rabbit mAb (A23795)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Mesothelin Rabbit mAb (A23795)

Western blot analysis of lysates from OVCAR3, using Mesothelin Rabbit mAb (A23795) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Mesothelin Rabbit mAb (A23795)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Mesothelin Rabbit mAb (A23795) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Mesothelin Rabbit mAb (A23795)

Immunohistochemistry analysis of paraffin-embedded Human mesothelioma tissue using Mesothelin Rabbit mAb (A23795) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Overview

Product nameMesothelin Rabbit mAb
Catalog No.A23795
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54389
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2).
SequenceEVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS
Gene ID
Swiss Prot
SynonymsMPF; SMRP; Mesothelin
Calculated MW67KDa/68KDa/71KDa
Observed MW40kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesOVCAR3 cells
Cellular locationCell membrane, GPI-anchor, Golgi apparatus, Lipid-anchor, Secreted.

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Lot number

ABclonal:Western blot - Mesothelin Rabbit mAb (A23795)}

Western blot - Mesothelin Rabbit mAb (A23795)

Western blot analysis of lysates from OVCAR3, using Mesothelin Rabbit mAb (A23795) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Mesothelin Rabbit mAb (A23795)}

Immunohistochemistry - Mesothelin Rabbit mAb (A23795)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Mesothelin Rabbit mAb (A23795) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Mesothelin Rabbit mAb (A23795)}

Immunohistochemistry - Mesothelin Rabbit mAb (A23795)

Immunohistochemistry analysis of paraffin-embedded Human mesothelioma tissue using Mesothelin Rabbit mAb (A23795) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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