Overview
Product name | Biotinylated Recombinant Human PDGFR-alpha/CD140a Protein |
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Catalog No. | RP02476B |
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Description | Biotinylated Recombinant Human PDGF R alpha / PDGFRA Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Glu524) of Human PDGF R alpha / PDGFRA fused with His tag and Avi tag at the C-terminal. |
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Purity | > 95% by Tris-Bis PAGE and HPLC |
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Endotoxin | < 1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 59.2 kDa |
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Background
Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
Product information
Synonym | PDGFRA; CD140A; PDGFR-2; PDGFR2; GAS9; RHEPDGFRA |
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Expression Host | HEK293 cells |
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Tag | C-His&Avi |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.