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Phospho-PDGFR alpha-Y849 Rabbit pAb (AP0569)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-PDGFR alpha-Y849 Rabbit pAb (AP0569)

Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y849 Rabbit pAb (AP0569) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-PDGFR alpha-Y849 Rabbit pAb
Catalog No.AP0569
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
ImmunogenA synthetic phosphorylated peptide around Y849 of human PDGFRA (NP_006197.1).
SequenceSNYVS
Gene ID
Swiss Prot
SynonymsCD140A; PDGFR2; PDGFR-2; Phospho-PDGFR alpha-Y849
Calculated MW123kDa
Observed MW190kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHep G2 treated by EGF
Cellular locationCell membrane, Single-pass type I membrane protein

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ABclonal:Western blot - Phospho-PDGFR alpha-Y849 Rabbit pAb (AP0569)}

Western blot - Phospho-PDGFR alpha-Y849 Rabbit pAb (AP0569)

Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y849 Rabbit pAb (AP0569) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)