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ABflo® 488 Rabbit anti-Human/Mouse PDGFRα/CD140a mAb (A22689)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Flow cytometry:1X10^6 HeLa cells (negative control, Left) and U-138 MG cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Mouse PDGFRα/CD140a mAb (A22689, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 NIH/3T3 cells were surface-stained with ABflo® 488 Rabbit anti-Mouse PDGFRα/CD140a mAb (A22689, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained NIH/3T3 cells were used as blank control (red line).

Flow cytometry:1X10^6 U-138 MG cells were surface-stained with ABflo™ 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo™ 488 Rabbit anti-Human/Mouse PDGFRα/CD140a mAb (A22689, 5 μl/Test, right).

Flow cytometry:1X10^6 NIH/3T3 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse PDGFRα/CD140a mAb (A22689, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human/Mouse PDGFRα/CD140a mAb
Catalog No.	A22689
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC56217-ABf488

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 24-524 of human PDGFRα (NP_006197.1).
Sequence	QLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSE
Gene ID
Swiss Prot
Synonyms	CD140A; PDGFR2; PDGFR-2
Calculated MW	24kDa/82kDa/123kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein