Overview
Product name | Biotinylated Recombinant Human P-Selectin/SELP/CD62P Protein |
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Catalog No. | RP02487B |
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Description | Biotinylated Recombinant Human P-Selectin/SELP/CD62P Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Trp42-Ala771) of Human P-Selectin/SELP/CD62P fused with His tag and Avi tag at the C-terminus. |
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Purity | > 95% by Tris-Bis PAGE and HPLC |
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Endotoxin | < 1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 82.9 kDa |
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Background
Human P-Selectin is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Product information
Synonym | CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL; CD62P/P-selectin; SELP; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL |
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Expression Host | HEK293 cells |
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Tag | C-His&Avi |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.