Recombinant Human P-Selectin/SELP/CD62P Protein (RP01397)

Overview

Product name	Recombinant Human P-Selectin/SELP/CD62P Protein
Catalog No.	RP01397
Description	Recombinant Human P-Selectin/SELP/CD62P Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Trp42-Ala771) of human P-Selectin/CD62P (Accession #NP_002996.2) fused with a Fc, 6×His tag at the C-terminus.
Bio-Activity	Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to Human SELP coated plates (10 μg/mL with 100 μL/well), > 80% cells will adhere after 1 hour at 37℃. 2.Measured by its binding ability in a functional ELISA.Immobilized PE Mouse Anti-Human CD62P Antibody at 1μg/mL (25 μL/well) can bind Human CD62P with a linear range of 0.46-7.82 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	<1EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Human
Calculated MW	106.75 kDa

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

Product information

Synonym	CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL; CD62P/P-selectin; SELP; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.