Overview
| Product name | Recombinant Human Alkaline phosphatase/tissue-nonspecific isozyme/ALPL Protein |
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| Catalog No. | RP01989 |
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| Description | Recombinant Human Alkaline phosphatase/tissue-nonspecific isozyme/ALPL Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu18-Ala500) of Human Alkaline phosphatase/tissue-nonspecific isozyme/ALPL (Accession #NP_000469.3) fused with His tag at the C-terminus. |
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| Purity | ≥ 90 % as determined by SDS-PAGE |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5. |
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| Species | Human |
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| Calculated MW | 54.04 kDa |
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Background
Four distinct genes encode alkaline phosphatases (APs) in humans . The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue‑nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers . There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones . The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor.
Product information
| Synonym | ALPL，Alkaline phosphatase; tissue-nonspecific isozyme; Alkaline phosphatase liver/bone/kidney isozyme; |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
