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Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)

Western blot analysis of lysates from HeLa cells, using Alkaline Phosphatase (ALPL) Rabbit pAb (A12396) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) Rabbit pAb (A12396) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameAlkaline Phosphatase (ALPL) Rabbit pAb
Catalog No.A12396
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 230-440 of human Alkaline Phosphatase (ALPL) (NP_000469.3).
SequenceNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNN
Gene ID
Swiss Prot
SynonymsHOPS; HPPA; HPPC; HPPI; HPPO; TNAP; TNALP; APTNAP; TNSALP; AP-TNAP; TNS-ALP; Alkaline Phosphatase (ALPL)
Calculated MW57kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationCell membrane, GPI-anchor, Lipid-anchor.
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)}

    Western blot - Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)

    Western blot analysis of lysates from HeLa cells, using Alkaline Phosphatase (ALPL) Rabbit pAb (A12396) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)}

    Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit pAb (A12396)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) Rabbit pAb (A12396) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12396? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)