Recombinant Human CSF-2/GM-CSF Protein (RP00094)

Overview

Product name	Recombinant Human CSF-2/GM-CSF Protein
Catalog No.	RP00094
Description	Recombinant Human CSF-2/GM-CSF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala18-Glu144) of human GM-CSF/CSF2 (Accession #NP_000749.2) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 30-140 pg/mL, corresponding to a specific activity of 7.14×10⁶-3.33×10⁷units/mg.
Purity	> 92% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	15.02 kDa

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

Product information

Synonym	CSF2; GMCSF
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.